Using a Microscope

Here is a diagram of a standard school light microscope, with an explanation of its parts and how to use them.

The eyepiece can be adjusted to focus the image properly in your eye. Sometimes there are two.
The lenses can be rotated to low power (x40), medium (x100), or high power (x400). Always start with the low power lens (the short one); move it as close to the slide as possible (as you watch from the side), using the coarse focusing knob.
The stage holds the glass slide containing the object to be viewed.
The aperture, or iris diaphragm, allows you to control the amount of light entering the lense.
The light source may be a bulb or a mirror. A mirror will have to be pointed at an external light source; never point it directly at the sun.
The coarse focus adjustment knob lets you quickly focus on the object to be viewed. Use this one first, and turn it so the lense moves upward.
The fine focus adjustment knob lets you adjust the focus so the image is sharply defined.
The handle. Use one hand here, and the other under the base, when you are carrying the microscope.


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